货号 | 名称 | 同裂酶 | 异裂酶 | 识别位点 | 切割末端 | 反应温度 | 失活条件 | 甲基化影响 |
NBS8183 | FastCut NcoI | Bsp19I |
| CCATGG 5'...C ↓ C A T G G...3' 3'...G G T A C ↑ C...5' | 5’ protruding ends | 37℃ | 80℃, 20 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect. |
NBS8184 | FastCut NdeI | FauNDI |
| CATATG 5'...C A ↓ T A T G...3' 3'...G T A T A ↑ C...5' | 5’ protruding ends | 37℃ | 80℃, 20 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect. |
NBS8185 | FastCut NheI | AsuNHI | BmtI, BspOI | GCTAGC 5'...G ↓ C T A G C...3' 3'...C G A T C ↑ G...5' | 5’ protruding ends | 37℃ | 80℃, 20 min | Dam: no effect. Dcm: no effect. CpG: some blocked. EcoKI: no effect. EcoBI: no effect. |
NBS8186 | FastCut NotI | CciNI |
| GCGGCCGC 5'...G C ↓ G G C C G C...3' 3'...C G C C G G ↑ C G...5' | 5’ protruding ends | 37℃ | 80℃, 20 min | Dam: no effect. Dcm: no effect. CpG: impaired. EcoKI: no effect. EcoBI: no effect. |
NBS8187 | FastCut NruI | Bsp68I， BtuMI， RruI |
| TCGCGA 5'...T C G ↓ C G A...3' 3'...A G C ↑ G C T...5' | blunt | 37℃ | No (column purification) | Dam: blocked. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect. |
NBS8188 | FastCut NsiI | EcoT22I, Mph1103I, Zsp2I |
| ATGCAT 5'...A T G C A ↓ T...3' 3'...T ↑ A C G T A...5' | 3’ protruding ends | 37℃ | 80℃, 20 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: some impaired. |
NBS8189 | FastCut PacI |
|
| TTAATTAA 5'...T T A A T ↓ T A A...3' 3'...A A T ↑ T A A T T...5' | 3’ protruding ends | 37℃ | 80℃, 20 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect. |
NBS8190 | FastCut PmeI | MssI |
| GTTTAAAC 5'...G T T T ↓ A A A C...3' 3'...C A A A ↑ T T T G...5' | blunt | 37℃ | 80℃,20 min | Dam: no effect. Dcm: no effect. CpG: some blocked. EcoKI: no effect. EcoBI: no effect. |
NBS8191 | FastCut PmlI | AcvI, Eco72I, PspCI, BbrPI |
| CACGTG 5'...CAC ↓ GTG...3' 3'...GTG ↑ CAC ..5' | blunt | 37℃ | 80℃，20 min | Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: some blocked. EcoBI: some blocked. |
NBS8192 | FastCut PspGI (EcoRII) | EcoRII, Psp6I, AjnI | BciT130I, BseBI, Bst2UI, BstNI, MvaI | CCWGG 5'...↓C C W G G ...3' 3'... G G W C C↑...5' | 5’ protruding ends | 75℃ | No (column purification) | Dam: no effect. Dcm: blocked. CpG: no effect. EcoKI: no effect. EcoBI: no effect. |
NBS8193 | FastCut PstI | BspMAI |
| CTGCAG 5'...C T G C A ↓ G...3' 3'...G ↑ A C G T C...5' | 3’ protruding ends | 37℃ | No (column purification) | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect. |
NBS8194 | FastCut PvuI | Ple19I |
| CGATCG 5'...C G A T ↓ C G...3' 3'...G C ↑ T A G C...5' | 3’ protruding ends | 37℃ | 80℃, 20 min | Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect. |
NBS8195 | FastCut PvuII |
|
| CAGCTG 5'...C A G ↓ C T G...3' 3'...G T C ↑ G A C...5' | blunt | 37℃ | No (column purification) | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: impaired. |
NBS8196 | FastCut RsaI | AfaI | Csp6I, CviQI, RsaNI | GTAC 5'...G T ↓ A C...3' 3'...C A ↑ T G...5' | blunt | 37℃ | No (column purification) | Dam: no effect. Dcm: no effect. CpG: impaired. EcoKI: no effect. EcoBI: no effect. |
NBS8197 | FastCut SacI | Psp124BI, SstI | Ecl136II, EcoICRI, Eco53kI | GAGCTC 5'...G A G C T ↓ C...3' 3'...C ↑ T C G A G...5' | 3’ protruding ends | 37℃ | 80℃, 20 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect. |
NBS8198 | FastCut SacII | Cfr42I， KspI， Sfr303I， SgrBI |
| CCGCGG 5'...C C G C ↓ G G...3' 3'...G G ↑ C G C C...5' | 3’ protruding ends | 37℃ | 80℃, 20 min | Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect. |
NBS8199 | FastCut SalI |
|
| GTCGAC 5'...G ↓ T C G A C...3' 3'...C A G C T ↑ G...5' | 5’ protruding ends | 37℃ | 80℃, 20 min | Dam: no effect. Dcm: no effect. CpG: some blocked. EcoKI: no effect. EcoBI: no effect. |
NBS8200 | FastCut SapI | BspQI, LguI, PciSI |
| GCTCTTC(1/4)
5'...G C T C T T C (N)₁↓...3' 3'...C G A G A A G (N)₄↑...5' | 5’ protruding ends | 37℃ | 80℃, 20 min | Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect. |
NBS8201 | FastCut SbfI | SdaI, Sse8387I |
| CCTGCAGG 5'...C C T G C A ↓ G G...3' 3'...G G ↑ A C G T C C...5' | 3’ protruding ends | 37℃ | 80℃, 20 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect. |
NBS8202 | FastCut ScaI | ZrmI, BmcAI |
| AGTACT 5'...A G T ↓ A C T...3' 3'...T C A ↑ T G A...5' | blunt | 37℃ | 80℃, 20 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: blocked. |
NBS8203 | FastCut SdaI (SbfI) | SbfI, Sse8387I |
| CCTGCAGG 5'...C C T G C A ↓ G G...3' 3'...G G ↑ A C G T C C...5' | 3’ protruding ends | 37℃ | 80℃, 20 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect. |
NBS8204 | FastCut SfiI |
|
| GGCCNNNNNGGCC 5'...G G C C N N N N ↓ N G G C C...3' 3'...C C G G N ↑ N N N N C C G G...5' | 3’ protruding ends | 50℃ | No (column purification) | Dam: no effect. Dcm: impaired. CpG: impaired. EcoKI: no effect. EcoBI: no effect. |
NBS8205 | FastCut SmaI |
| Cfr9I, TspMI, XmaI | CCCGGG 5'...C C C ↓ G G G...3' 3'...G G G ↑ C C C...5' | blunt | 25℃ | 80℃, 20 min | Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect. |
NBS8206 | FastCut SnaBI | Eco105I, BstSNI |
| TACGTA 5'...T A C ↓ G T A...3' 3'...A T G ↑ C A T...5' | blunt | 37℃ | 80℃, 20 min | Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect. |
NBS8207 | FastCut SpeI | AhlI， BcuI |
| ACTAGT 5'...A ↓ C T A G T...3' 3'...T G A T C ↑ A...5' | 5’ protruding ends | 37℃ | 80℃, 20 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: blocked. EcoBI: blocked. |
NBS8208 | FastCut SphI | PaeI |
| GCATGC 5'...G C A T G ↓ C...3' 3'...C ↑ G T A C G...5' | 3’ protruding ends | 37℃ | 80℃, 20 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: blocked. |
NBS8209 | FastCut SspI |
|
| AATATT 5'...A A T ↓ A T T...3' 3'...T T A ↑ T A A...5' | blunt | 37℃ | 80℃, 20 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect. |
NBS8210 | FastCut StuI | Eco147I, PceI, SseBI |
| AGGCCT 5'...A G G ↓ C C T...3' 3'...T C C ↑ G G A...5' | blunt | 37℃ | 80℃, 20 min | Dam: no effect. Dcm: blocked. CpG: no effect. EcoKI: no effect. EcoBI: no effect. |
NBS8211 | FastCut TaqI |
|
| TCGA 5'...T ↓ C G A...3' 3'...A G C ↑ T...5' | 5’ protruding ends | 65℃ | No (column purification) | Dam: blocked. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect. |
NBS8212 | FastCut Tsp45I | NmuCI, TseFI |
| GTSAC 5'...↓G T S A C ...3' 3' ... C A S T G↑...5' | 5’ protruding ends | 65℃ | No (column purification) | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: blocked. |
NBS8213 | FastCut XbaI |
|
| TCTAGA 5'...T ↓ C T A G A...3' 3'...A G A T C ↑ T...5' | 5’ protruding ends | 37℃ | 80℃, 20 min | Dam: blocked. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect. |
NBS8214 | FastCut XcmI |
|
| CCANNNNNNNNNTGG 5'...C C A N N N N N ↓ N N N N T G G...3' 3'...G G T N N N N ↑ N N N N N A C C...5' | 3’ protruding ends | 37℃ | 80℃，20 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect. |
NBS8215 | FastCut XhoI | PaeR7I, Sfr274I, SlaI |
| CTCGAG 5'...C ↓ T C G A G...3' 3'...G A G C T ↑ C...5' | 5’ protruding ends | 37℃ | 80℃, 20 min | Dam: no effect. Dcm: no effect. CpG: impaired. EcoKI: no effect. EcoBI: no effect. |
NBS8216 | AarI | PaqCI |
| CACCTGC(4/8) 5'...C A C C T G C (N)₄↓...3' 3'...G T G G A C G (N)₈↑...5' | 5’ protruding ends | 37℃ | 80℃, 20 min | Dam: no effect. Dcm: no effect. CpG: impaired. EcoKI: no effect. EcoBI: no effect. |
NBS8217 | ApeKI | TseI |
| GCWGC 5'...G↓C W G C...3' 3'...C G W C↑G ...5' | 5’ protruding ends | 75℃ | No (column purification) | Dam: no effect. Dcm: no effect. CpG: some blocked. EcoKI: no effect. EcoBI: no effect. |
NBS8218 | BbvCI |
|
| CCTCAGC 5'...C C↓T C A G C...3' 3'...G G A G T↑C G ...5' | 5’ protruding ends | 37℃ | 80℃, 20 min | Dam: no effect. Dcm: no effect. CpG: impaired. EcoKI: no effect. EcoBI: no effect. |
NBS8219 | BpiI | BstV2I, BbsI |
| GAAGAC(2/6) 5'...G A A G A C (N)₂↓...3' 3'...C T T C T G (N)₆↑...5' | 5’ protruding ends | 37℃ | 80℃, 20 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect. |
NBS8220 | BsiWI | PspLI, Pfl23II |
| CGTACG 5'...C ↓ G T A C G...3' 3'...G C A T G ↑ C...5' | 5’ protruding ends | 55℃ | 80℃, 20 min | Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect. |
NBS8221 | BsmBI | Esp3I |
| CGTCTC(1/5) 5'...C G T C T C (N)₁↓...3' 3'...G C A G A G (N)₅↑...5' | 5’ protruding ends | 55℃ | 80℃, 20 min | Dam: no effect. Dcm: no effect. CpG: some blocked. EcoKI: no effect. EcoBI: impaired. |
NBS8222 | BspQI | SapI, LguI, PciSI |
| GCTCTTC(1/4) 5'...G C T C T T C (N)₁↓...3' 3'...C G A G A A G (N)₄↑...5' | 5’ protruding ends | 50℃ | 80℃, 20 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect. |
NBS8223 | BsrDI | Bse3DI, BseMI |
| GCAATGNN 5'...G C A A T G N N ↓...3' 3'...C G T T A C↑N N ...5' | 3’ protruding ends | 37℃ | 80℃, 20 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: some blocked. |
NBS8224 | BstXI |
|
| CCANNNNNNTGG 5'...C C A N N N N N ↓ N T G G...3' 3'...G G T N ↑ N N N N N A C C...5' | 3’ protruding ends | 37℃ | 80℃, 20 min | Dam: no effect. Dcm: impaired. CpG: no effect. EcoKI: no effect. EcoBI: no effect. |
NBS8225 | PciI | PscI |
| ACATGT 5'...A ↓ C A T G T...3' 3'...T G T A C ↑ A...5' | 5’ protruding ends | 37℃ | 80℃, 20 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: some blocked. EcoBI: some blocked. |
NBS8226 | Sgel |
|
| ᵐ⁵CNNG(9/13) 5'...ᵐ⁵ C N N G(N)₉ ↓...3' 3'... G N N C(N)₁₃ ↑...5'* *SgeI 可识别与切割含有5-mC位点的DNA靶标序列，一条链或双链甲基化均可被识别。 | 3’ protruding ends | 37℃ | 80℃, 20 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect. |
NBS8227 | SgrAI |
|
| CRCCGGYG 5'...C R ↓ C C G G Y G...3' 3'...G Y G G C C ↑ R C...5' | 5’ protruding ends | 37℃ | 80℃, 20 min | Dam: no effect. Dcm: no effect. CpG: impaired. EcoKI: some blocked. EcoBI: some blocked. |
NBS8228 | SspDI (KasI) | KasI | DinI, EgeI, EheI, Mly113I, NarI, PluTI, SfoI | GGCGCC 5'...G ↓ G C G C C...3' 3'...C C G C G ↑ G...5' | 5’ protruding ends | 37℃ | 80℃, 20 min | Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect. |
NBS8229 | SwaI | SmiI |
| ATTTAAAT 5'...A T T T↓A A A T...3' 3'...T A A A↑T T T A ...5' | blunt | 37℃ | 80℃, 20 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect. |
NBS8230 | XmnI | PdmI, MroXI |
| GAANNNNTTC 5'...G A A N N↓N N T T C...3' 3'...C T T N N↑N N A A G ...5' | blunt | 37℃ | 80℃, 20 min | Dam: no effect. Dcm: no effect. CpG: impaired. EcoKI: no effect. EcoBI: blocked. |
NBS8231 | Esp3I(BsmBI), ADCF | BsmBI |
| CGTCTC(1/5) 5'...C G T C T C (N)₁↓...3' 3'...G C A G A G (N)₅↑...5' | 5’ protruding ends | 37℃ | 80℃, 20 min | Dam: no effect. Dcm: no effect. CpG: impaired. EcoKI: no effect. EcoBI: no effect. |
NBS8232 | EcoRI, ADCF | FunII |
| GAATTC 5'...G ↓ A A T T C...3' 3'...C T T A A ↑ G...5' | 5’ protruding ends | 37℃ | 80℃, 20 min | Dam: no effect. Dcm: no effect. CpG: impaired. EcoKI: no effect. EcoBI: impaired. |
NBS8233 | Nb.BbvCI |
|
| CCTCAGC 5'...C C T C A G C...3' 3'...G G A G T↑C G...5' |
| 37℃ | 80℃, 20 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: blocked. |
NBS8234 | Nb.BsrDI |
|
| GCAATGNN 5'...G C A A T G N N...3' 3'...C G T T A C↑N N ...5' |
| 65℃ | 80℃, 20 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: impaired. |
NBS8235 | Nt.BbvCI |
|
| CCTCAGC 5'...C C↓T C A G C...3' 3'...G G A G T C G...5' |
| 37℃ | 80℃, 20 min | Dam: no effect. Dcm: no effect. CpG: impaired. EcoKI: no effect. EcoBI: blocked. |
NBS8236 | Nt.BspQI |
|
| GCTCTTCN 5'...G C T C T T C N↓...3' 3'...C G A G A A G N ...5' |
| 50℃ | 80℃, 20 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: blocked. |
NBS8237 | Nt.BstNBI |
|
| GAGTCNNNNN 5'...G A G T C N N N N↓N...3' 3'...C T C A G N N N N N ...5' |
| 55℃ | 80℃, 20 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect. |
NBS8238 | MazF |
|
| ACA 5'...↓ A C A ...3' | 5’ protruding ends | 37℃ |
|
|
NBS8239 | RNase GG |
|
| GG 5'...G ↓ G ...3' |
| 37℃ |
|
|
NBS8240 | I-SceI |
|
| TAGGGATAACAGGGTAAT 5'...T A G G G A T A A ↓ C A G G G T A A T...3' 3'...A T C C C ↑ T A T T G T C C C A T T A...5' | 3’ protruding ends | 37℃ | 80℃, 20 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect. |
