货号 | 名称 | 同裂酶 | 异裂酶 | 识别位点 | 切割末端 | 反应 温度 | 失活条件 | 甲基化影响 |
NBS8129 | FastCut AatII |
| ZraI | GACGTC 5'...G A C G T ↓ C...3' 3'...C ↑ T G C A G...5' | 3’protruding ends | 37℃ | 80℃, 20 min | Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect. |
NBS8130 | FastCut AccI | XmiI, FblI |
| GTMKAC 5'...G T ↓ M K A C...3' 3'...C A K M ↑ T G...5' | 5’protruding ends | 37℃ | 80℃, 20 min | Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect. |
NBS8131 | FastCut AciI | BspACI, SsiI |
| CCGC 5'...C ↓ C G C...3' 3'...G G C ↑ G...5' | 5’protruding ends | 37℃ | 80℃, 20 min | Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect. |
NBS8132 | FastCut AflII | BspTI, BfrI, BstAFI, MspCI, Vha464I |
| CTTAAG 5'...C ↓ T T A A G...3' 3'...G A A T T ↑ C...5' | 5’protruding ends | 37℃ | 80℃, 20 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect. |
NBS8133 | FastCut AgeI | AsiGI,
CspAI, BshTI, PinAI |
| ACCGGT 5'...A ↓ C C G G T...3' 3'...T G G C C ↑ A...5' | 5’protruding ends | 37℃ | 80℃, 20 min | Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect. |
NBS8134 | FastCut AluI | AluBI |
| AGCT 5'...A G ↓ C T...3' 3'...T C ↑ G A...5' | blunt | 37℃ | 80℃, 20 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: blocked. |
NBS8135 | FastCut ApaI | Bsp120I, PspOMI |
| GGGCCC 5'...G G G C C ↓ C...3' 3'...C ↑ C C G G G...5' | 3’protruding ends | 37℃ | 80℃, 20 min | Dam: no effect. Dcm: some blocked. CpG: some blocked. EcoKI: no effect. EcoBI: no effect. |
NBS8136 | FastCut ApaLI | Alw44I, VneI |
| GTGCAC 5'...G ↓ T G C A C...3' 3'...C A C G T ↑ G...5' | 5’protruding ends | 37℃ | 80℃, 20 min | Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: impaired. EcoBI: no effect. |
NBS8137 | FastCut AscI | PalAI, SgsI |
| GGCGCGCC 5'...G G ↓ C G C G C C...3' 3'...C C G C G C ↑ G G...5' | 5’protruding ends | 37℃ | 80℃, 20 min | Dam: no effect. Dcm: no effect. CpG: impaired. EcoKI: no effect. EcoBI: no effect. |
NBS8138 | FastCut AseI | PshBI, VspI |
| ATTAAT 5'...A T ↓ T A A T...3' 3'...T A A T ↑ T A...5' | 5’protruding ends | 37℃ | 80℃, 20 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: impaired. |
NBS8139 | FastCut AvaII | Bme18I, Eco47I |
| GGWCC 5'...G ↓ G W C C...3' 3'...C C W G ↑ G...5' | 5’protruding ends | 37℃ | 80℃, 20 min | Dam: no effect. Dcm: blocked. CpG: blocked. EcoKI: no effect. EcoBI: no effect. |
NBS8140 | FastCut AvrII | AspA2I, BlnI, XmaJI |
| CCTAGG 5'...C ↓ C T A G G...3' 3'...G G A T C ↑ C...5' | 5’protruding ends | 37℃ | No (column purification) | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect. |
NBS8141 | FastCut BamHI |
|
| GGATCC 5'...G ↓ G A T C C...3' 3'...C C T A G ↑ G...5' | 5’protruding ends | 37℃ | No (column purification) | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect. |
NBS8142 | FastCut BbsI | BstV2I, BpiI |
| GAAGAC(2/6) 5'...G A A G A C (N)₂↓...3' 3'...C T T C T G (N)₆↑...5' | 5’protruding ends | 37℃ | 80℃, 20 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect. |
NBS8143 | FastCut BclI | FbaI, Ksp22I |
| TGATCA 5'...T ↓ G A T C A...3' 3'...A C T A G ↑ T...5' | 5’protruding ends | 37℃ | 80℃, 20 min | Dam: blocked. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: some blocked. |
NBS8144 | FastCut BglI |
|
| GCCNNNNNGGC 5'...G C C N N N N ↓ N G G C...3' 3'...C G G N ↑ N N N N C C G...5' | 5’protruding ends | 37℃ | 80℃, 20 min | Dam: no effect. Dcm: no effect. CpG: impaired. EcoKI: no effect. EcoBI: no effect. |
NBS8145 | FastCut BglII |
|
| AGATCT 5'...A ↓ G A T C T...3' 3'...T C T A G ↑ A...5' | 5’protruding ends | 37℃ | No (column purification) | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: some blocked. |
NBS8146 | FastCut BsaI | Eco31I, Bso31I, BspTNI |
| GGTCTC(1/5) 5'...G G T C T C (N)₁ ↓...3' 3'...C C A G A G (N)₅ ↑...5' | 5’protruding ends | 37℃ | 80℃, 20 min | Dam: no effect. Dcm: impaired. CpG: impaired. EcoKI: no effect. EcoBI: no effect. |
NBS8147 | FastCut Bsh1236I (BstUI) | BstUI, BspFNI, BstFNI, AccII |
| CGCG 5'...C G ↓ C G...3' 3'...G C ↑ G C...5' | blunt | 37℃ | 80℃, 20 min | Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect. |
NBS8148 | FastCut BsmI | BsaMI, Mva1269I, PctI |
| GAATGCN 5'...G A A T G C N ↓...3' 3'...C T T A C↑G N ...5' | 5’protruding ends | 37℃ | 80℃, 20 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: blocked. |
NBS8149 | FastCut BspHI | CciI, PagI |
| TCATGA 5'...T ↓ C A T G A...3' 3'...A G T A C ↑ T...5' | 5’protruding ends | 37℃ | 80℃, 20 min | Dam: impaired. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: impaired. |
NBS8150 | FastCut BsrGI | BstAUI, Bsp1407I |
| TGTACA 5'...T ↓ G T A C A...3' 3'...A C A T G ↑ T...5' | 5’protruding ends | 37℃ | 80℃, 20 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect. |
NBS8151 | FastCut BstBI | AsuII, Bpu14I, Bsp119I, BspT104I, NspV, SfuI |
| TTCGAA 5'...T T ↓ C G A A...3' 3'...A A G C ↑ T T...5' | 5’protruding ends | 37℃ | 80℃, 20 min | Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: some blocked. EcoBI: no effect. |
NBS8152 | FastCut BstEII | BstPI， Eco91I， EcoO65I， PspEI |
| GGTNACC 5'...G ↓ G T N A C C...3' 3'...C C A N T G ↑ G...5' | 5’protruding ends | 37℃ | 80℃, 20 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: impaired. EcoBI: impaired. |
NBS8153 | FastCut Cfr42I (SacII) | SacII， KspI， Sfr303I， SgrBI |
| CCGCGG 5'...C C G C ↓ G G...3' 3'...G G ↑ C G C C...5' | 3’protruding ends | 37℃ | 80℃, 20 min | Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect. |
NBS8154 | FastCut ClaI | BspDI， Bsa29I， BseCI， BshVI， Bsu15I， BsuTUI |
| ATCGAT 5'...A T ↓ C G A T...3' 3'...T A G C ↑ T A...5' | 5’protruding ends | 37℃ | 80℃, 20 min | Dam: blocked. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: impaired. |
NBS8155 | FastCut DdeI | BstDEI, HpyF3I |
| CTNAG 5'...C ↓ T N A G...3' 3'...G A N T ↑ C...5' | 5’protruding ends | 37℃ | 80℃, 20 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: blocked. |
NBS8156 | FastCut DpnI | MalI | Bsp143I, BssMI, BstKTI, BstMBI, DpnII, Kzo9I, MboI, NdeII, Sau3AI | GAᵐ⁶TC 5'...G Aᵐ⁶ ↓ T C...3' 3'...C T ↑ Aᵐ⁶ G...5' | blunt | 37℃ | 80℃, 20 min | Dam: no effect. Dcm: no effect. CpG: impaired. EcoKI: no effect. EcoBI: impaired. |
NBS8157 | FastCut DpnII | MboI， Sau3AI， Bsp143I, BssMI， BstMBI， Kzo9I， NdeII | BstKTI, DpnI, MalI | GATC 5'...↓G A T C...3' 3' ... C T A G↑...5' | 5’protruding ends | 37℃ | 80℃, 20 min | Dam: blocked. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: blocked. |
NBS8158 | FastCut DraI |
|
| TTTAAA 5'...T T T ↓ A A A...3' 3'...A A A ↑ T T T ..5' | blunt | 37℃ | 80℃，20 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: blocked. EcoBI: no effect. |
NBS8159 | FastCut DraIII | AdeI |
| CACNNNGTG 5'...C A C N N N ↓ G T G...3' 3'...G T G ↑ N N N C A C...5' | 5’protruding ends | 37℃ | No (column purification) | Dam: no effect. Dcm: no effect. CpG: some impaired. EcoKI: impaired. EcoBI: some blocked. |
NBS8160 | FastCut EagI | BseX3I, BstZI, EclXI, Eco52I |
| CGGCCG 5'...C ↓ G G C C G...3' 3'...G C C G G ↑ C ..5' | 5’protruding ends | 37℃ | 80℃, 20 min | Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect. |
NBS8161 | FastCut EarI | Eam1104I, Bst6I |
| CTCTTC(1/4) 5'...C T C T T C (N)₁↓...3' 3'...G A G A A G (N)₄↑...5' | 5’protruding ends | 37℃ | 80℃, 20 min | Dam: no effect. Dcm: no effect. CpG: impaired. EcoKI: no effect. EcoBI: no effect. |
NBS8162 | FastCut EcoRI | FunII |
| GAATTC 5'...G ↓ A A T T C...3' 3'...C T T A A ↑ G...5' | 5’protruding ends | 37℃ | 80℃, 20 min | Dam: no effect. Dcm: no effect. CpG: impaired. EcoKI: no effect. EcoBI: impaired. |
NBS8163 | FastCut EcoRV | Eco32I |
| GATATC 5'...G A T ↓ A T C...3' 3'...C T A ↑ T A G...5' | blunt | 37℃ | 80℃, 20 min | Dam: no effect. Dcm: no effect. CpG: some impaired. EcoKI: no effect. EcoBI: some blocked. |
NBS8164 | FastCut Esp3I (BsmBI) | BsmBI |
| CGTCTC(1/5) 5'...C G T C T C (N)₁↓...3' 3'...G C A G A G (N)₅↑...5' | 5’protruding ends | 37℃ | 80℃, 20 min | Dam: no effect. Dcm: no effect. CpG: impaired. EcoKI: no effect. EcoBI: no effect. |
NBS8165 | FastCut FokI |
| BtsCI, BstF5I, BseGI | GGATG(9/13) 5'...G G A T G (N)₉ ↓...3' 3'...C C T A C (N)₁₃↑...5' | 5’protruding ends | 37℃ | 80℃, 20 min | Dam: no effect. Dcm: some impaired. CpG: some impaired. EcoKI: no effect. EcoBI: impaired. |
NBS8166 | FastCut FspI | Acc16I, NsbI |
| TGCGCA 5'...T G C ↓ G C A...3' 3'...A C G ↑ C G T...5' | blunt | 37℃ | No (column purification) | Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect. |
NBS8167 | FastCut HaeIII | BsuRI, BshFI, BspANI, BsnI | AoxI | GGCC 5'...G G ↓ C C...3' 3'...C C ↑ G G...5' | blunt | 37℃ | 80℃, 20 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect. |
NBS8168 | FastCut HhaI | HinP1I, CfoI, AspLEI, Hin6I, HspAI, BstHHI |
| GCGC 5'...G C G ↓ C...3' 3'...C ↑ G C G...5' | 3’ protruding ends | 37℃ | 80℃, 20 min | Dam: no effect. Dcm: no effect. CpG: impaired. EcoKI: no effect. EcoBI: no effect. |
NBS8169 | FastCut HindIII |
|
| AAGCTT 5'...A ↓ A G C T T...3' 3'...T T C G A ↑ A...5' | 5’ protruding ends | 37℃ | 80℃, 20 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: some blocked. |
NBS8170 | FastCut HinfI |
|
| GANTC 5'...G ↓ A N T C...3' 3'...C T N A ↑ G...5' | 5’ protruding ends | 37℃ | 80℃, 20 min | Dam: no effect. Dcm: no effect. CpG: impaired. EcoKI: no effect. EcoBI: blocked. |
NBS8171 | FastCut HinP1I | HhaI, CfoI, AspLEI, Hin6I, HspAI, BstHHI |
| GCGC 5'...G ↓ C G C...3' 3'...C G C ↑ G...5' | 5’ protruding ends | 37℃ | 80℃, 20 min | Dam: no effect. Dcm: no effect. CpG: impaired. EcoKI: no effect. EcoBI: no effect. |
NBS8172 | FastCut HpaI | KspAI |
| GTTAAC 5'...G T T ↓ A A C...3' 3'...C A A ↑ T T G...5' | blunt | 37℃ | 80℃, 20 min | Dam: no effect. Dcm: no effect. CpG: impaired. EcoKI: impaired. EcoBI: no effect. |
NBS8173 | FastCut HpaII | MspI, HapII, BsiSI |
| CCGG 5'...C ↓ C G G...3' 3'...G G C ↑ C...5' | 5’ protruding ends | 37℃ | 80℃, 20 min | Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: no effect. EcoBI: no effect. |
NBS8174 | FastCut KasI | SspDI | Mly113I, EgeI, NarI, EheI, DinI, SfoI, PluTI | GGCGCC 5'...G ↓ G C G C C...3' 3'...C C G C G ↑ G...5' | blunt | 37℃ | 80℃, 20 min | Dam: no effect. Dcm: no effect. CpG: impaired. EcoKI: no effect. EcoBI: no effect. Dam: no effect. |
NBS8175 | FastCut KpnI |
| Asp718I, Acc65I | GGTACC 5'...G G T A C ↓ C...3' 3'...C ↑ C A T G G...5' | 3’ protruding ends | 37℃ | No (column purification) | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect. |
NBS8176 | FastCut MboI | Bsp143I, Kzo9I, BssMI, DpnII, BstMBI, NdeII, Sau3AI | BstKTI | GATC 5'...↓G A T C ...3' 3'... C T A G↑...5' | 5’ protruding ends | 37℃ | 80℃, 20 min | Dam: blocked. Dcm: no effect. CpG: some impaired. EcoKI: no effect. EcoBI: some blocked. |
NBS8177 | FastCut MluI |
|
| ACGCGT 5'...A ↓ C G C G T...3' 3'...T G C G C ↑ A...5' | 5’ protruding ends | 37℃ | 80℃, 20 min | Dam: no effect. Dcm: no effect. CpG: blocked. EcoKI: impaired. EcoBI: impaired. |
NBS8178 | FastCut MnlI |
|
| CCTC(7/6) 5'...C C T C N₇ ↓...3' 3'...G G A G N₆ ↑...5' | 3’ protruding ends | 37℃ | 80℃, 20 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: blocked. |
NBS8179 | FastCut MscI | BalI, MlsI, Mox20I, Msp20I |
| TGGCCA 5'...T G G ↓ C C A...3' 3'...A C C ↑ G G T...5' | blunt | 37℃ | 80℃, 20 min | Dam: no effect. Dcm: blocked. CpG: no effect. EcoKI: no effect. EcoBI: no effect. |
NBS8180 | FastCut MseI | SaqAI, Tru9I, Tru1I |
| TTAA 5'...T ↓ T A A...3' 3'...A A T ↑ T...5' | 5’ protruding ends | 37℃ | 80℃, 20 min | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: some blocked. EcoBI: no effect. |
NBS8181 | FastCut MspI | HpaII, HapII, BsiSI |
| CCGG 5'...C ↓ C G G...3' 3'...G G C ↑ C...5' | 5’ protruding ends | 37℃ | No (column purification) | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect. |
NBS8182 | FastCut MunI (MfeI) | MfeI |
| CAATTG 5'...C ↓ A A T T G...3' 3'...G T T A A ↑ C...5' | 5’ protruding ends | 37℃ | No (column purification) | Dam: no effect. Dcm: no effect. CpG: no effect. EcoKI: no effect. EcoBI: no effect. |
