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该试剂盒专门设计快速地从培养过夜的细菌中纯化低内毒素的质粒DNA，内毒素含量小于0.1EU/ug。纯化的质粒可直接用于测序、细胞转染、动物注射、酶切等各种用途。质粒的产量依赖于质粒的拷贝数、E.coli菌株以及生长条件，对于低拷贝菌株一次操作量可以高达100mL。 -
内毒素(Endotoxin)是革兰氏阴性菌细胞壁上的一种脂多糖，它能引起哺乳动物发热，甚至会导致动物休克。常规的质粒纯化试剂盒由于无法去除内毒素的污染，从而大大降低了质粒转染的效率。Omega公司采用独特的内毒素吸附缓冲液(ETR Binding Buffer)，能有效去除质粒中的内毒素污染，纯化的质粒中内毒素含量小于0.1EU/µg，可大大提高转染实验效率和动物注射效果。
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无内毒素超大量提取试剂盒可快速、高效地从大体积细菌中获得无内毒素质粒DNA。细胞裂解后，使用Buffer ETR去除上清中的内毒素。接下来的硅胶柱步骤纯化质粒DNA。
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The E.Z.N.A.® Endo-Free Plasmid DNA Maxi Kit provides an efficient method for the large-scale isolation of low-endotoxin plasmid DNA. The system utilizes Lysate Clearance Filter Syringes instead of centrifugation to rapidly clear bacterial lysates post-alkaline lysis. -
The E.Z.N.A.® Endo-Free Plasmid DNA Midi Kit provides an efficient method for the large-scale isolation of low-endotoxin plasmid DNA. The system utilizes Lysate Clearance Filter Syringes instead of centrifugation to rapidly clear bacterial lysates post-alkaline lysis. -
Plasmid isolated with traditional purification procedures normally contain high levels of endotoxins (also known as lipopolysaccharides or LPS) that can significantly interfere with transfection experiments downstream. -
Omega公司采用独特的内毒素吸附缓冲液(ETR Solution)，能有效去除质粒中的内毒素污染，纯化的质粒中内毒素含量小于0.1EU/µg，可大大提高转染实验效率和动物注射效果。
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The E.Z.N.A.® FastFilter Plasmid DNA Mini Kit is designed for rapid purification of high-quality plasmid DNA from up to 5 mL of culture following the alkaline-lysis method. The kit features an innovative and first-of-its-kind FastFilter Mini Column nested within a regular HiBind® DNA Mini Column to combine lysate clearance and DNA binding into one or two centrifugation steps depending on the culture volume. -
快速过滤质粒超大量提取试剂盒使用创新型方法，可以在40分钟内快速有效地纯化出2.5 mg高纯度质粒。该试剂盒使用过滤器可以快速过滤细胞裂解液。
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The E.Z.N.A.® FastFilter Plasmid DNA Maxi Kit combines the convenience of spin columns with the speed of syringe filters to ease large-scale plasmid preparations. The system utilizes Lysate Clearance Filter Syringes instead of centrifugation to rapidly clear bacterial lysates post-alkaline lysis and follows a simple “bind-wash-elute” procedure to deliver high-quality plasmid DNA.
公司名称：上海诺宁生物科技有限公司
联系Q Q：306458042（经销商）392324729（终端用户） 
联系微信：NoninBioTech（经销商）shnoninbio（终端用户）
联系地址：上海市闵行区虹梅南路2588号绿亮科创园A531
联系电话：021-61241614 18616364863
E-mail：noninbio@163.com
