<D6924> Fastfilter Plasmid DNA Maxi Kit 快速法质粒大量提取试剂盒

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快速法质粒大量提取试剂盒

Fastfilter Plasmid DNA Maxi Kit

产品编号	产品名称	包装规格	目录价
D6924-01	快速法质粒大量提取试剂盒	5T	405
D6924-03	快速法质粒大量提取试剂盒	25T	1900
D6924-04	快速法质粒大量提取试剂盒	100T	7200

产品介绍

The E.Z.N.A.® FastFilter Plasmid DNA Maxi Kit combines the convenience of spin columns with the speed of syringe filters to ease large-scale plasmid preparations. The system utilizes Lysate Clearance Filter Syringes instead of centrifugation to rapidly clear bacterial lysates post-alkaline lysis and follows a simple “bind-wash-elute” procedure to deliver high-quality plasmid DNA. The yields vary according to plasmid copy number, E. coli strain, and growth conditions. 50-200 mL of bacterial cultures in LB medium typically produces 0.6-1.2 mg of high copy number plasmid DNA. Up to 500 mL of culture may be processed when working with low copy number plasmid. The system uses centrifugation or vacuum technology for plasmid purification and eliminates the time-consuming gravity-flow columns that require alcohol precipitation. Purified plasmid DNA is suitable for automated fluorescent DNA sequencing (typical reads exceed 800 bp), restriction enzyme digestion, ligation, PCR, in vitro transcription, transformation, and other applications.

参数

FEATURES	SPECIFICATIONS
Downstream Application	Cloning, sequencing, transformation, PCR, restriction digestion, ligation, in vitro transcription etc.
Starting material	50–200 mL LB culture with OD600 between 2 and 3; or equivalent
Plasmid type	High-copy, low-copy, cosmid DNA
Processing mode	Manual (centrifugation or vacuum)
Throughput	1–24
DNA binding technology	Silica Maxi Spin Column
Lysate clearance method	Filtration using Lysate clearance syringe
Processing time	40 minutes
Yield	600–1200 μg for high copy-number; 50–300 μg for low copy-number
Special note	Lysate clearance syringe for rapid sample processing

组分

FastFilter Plasmid Maxi Kit	D6924-00	D6924-01	D6924-03	D6924-04
Purifications	2	5	25	100
HiBind® DNA Maxi Columns	2	5	25	100
50 mL Collection Tubes	2	5	25	100
Lysate Clearance Filter Syringe	2	5	25	100
Solution I	30 mL	60 mL	270 mL	1050 mL
Solution II	30 mL	60 mL	270 mL	1050 mL
N3 Buffer	15 mL	30 mL	135 mL	530 mL
GBT Buffer	20 mL	50 mL	230 mL	900 mL
HBC Buffer	18 mL	40 mL	200 mL	3 × 250 mL
DNA Wash Buffer	15 mL	40 mL	150 mL	3 × 200 mL
RNase A	120 μL	300 μL	1.2 mL	5 mL
Elution Buffer	15 mL	40 mL	150 mL	2 × 300 mL
User Manual	✓	✓	✓	✓

流程

Centrifugation Protocol

Pellet by Centrifugation
Resuspend, Lyse, Neutralize
Add GBT Lysis Buffer
Transfer Lysate to Lysate Clearance Syringe
Insert Plunger into Syringe & Clear Lysate into Centrifuge Tube
Pass Lysate through Column
Wash 3X
Transfer Column to Collection Tube & Centrifuge to Dry HiBind® matrix
Transfer Column to new Centrifuge Tube & Centrifuge to Elute DNA

Vacuum Protocol

Pellet by Centrifugation
Resuspend, Lyse, Neutralize
Add GBT Lysis Buffer
Transfer Lysate to Lysate Clearance Syringe
Insert Plunger into Syringe & Clear Lysate into Centrifuge Tube
Prepare Vacuum Manifold
Insert Column into Luer Connector on Vacuum Manifold
Pass Lysate through Column
Wash 3X
Transfer Column to Collection Tube & Centrifuge to Dry HiBind® matrix
Transfer Column to new Centrifuge Tube & Centrifuge to Elute DNA

上一篇： <D6929> Fastfilter Plasmid Mega Kit 快速法质粒超大量提取试剂盒

下一篇： <D6905> Fastfilter Plasmid DNA Midi Kit 快速法质粒中量提取试剂盒

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