<D6944> FastFilter Plasmid DNA Mini Kit 极速质粒小提试剂盒

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极速质粒小提试剂盒

FastFilter Plasmid DNA Mini Kit

产品编号	产品名称	包装规格	目录价
D6944-01	极速质粒小提试剂盒	50T	340
D6944-02	极速质粒小提试剂盒	200T	1200

产品介绍

The E.Z.N.A.® FastFilter Plasmid DNA Mini Kit is designed for rapid purification of high-quality plasmid DNA from up to 5 mL of culture following the alkaline-lysis method. The kit features an innovative and first-of-its-kind FastFilter Mini Column nested within a regular HiBind® DNA Mini Column to combine lysate clearance and DNA binding into one or two centrifugation steps depending on the culture volume. The protocol time is just 9 minutes when starting with bacterial pellet from up to 3 mL culture volume. The protocol time for culture volumes greater than 3 mL will be slightly longer due to two centrifugation steps for lysate clearance. Following the wash and elute steps, purified plasmid DNA is immediately ready for various downstream applications such as routine screening, restriction enzyme digestion, transformation, PCR, and DNA sequencing.

Typically, up to 5 mL overnight bacterial culture in LB or other suitable growth medium is centrifuged to pellet the bacterial cells. The cells are then lysed under alkaline conditions and subsequently neutralized to form white flocculent precipitate. Neutralized lysate is then transferred to a FastFilter Mini Column nested within a HiBind® DNA Mini Column. Upon adjustment of binding conditions, the lysate is centrifuged through this FastFilter HiBind® configuration to both clear the lysate and facilitate binding of DNA to the HiBind®
DNA Mini Column. The FastFilter Mini Column is discarded, and the HiBind® DNA Mini Column is subjected to two wash steps to remove salt and other contaminants. High-quality plasmid DNA is then eluted in low-salt elution buffer and is ready for use in a wide range of downstream applications mentioned above.

参数

FEATURES	SPECIFICATIONS
Starting Material	Bacteria harboring high-copy or low-copy plasmid in LB culture medium
Starting Amount	1–5 mL LB culture
Elution Volume	50–100 μL
Processing mode	Manual (centrifugation or vacuum)
Throughput	1–24
DNA binding technology	Silica Mini Spin Column
Processing time	9 minutes
Downstream Applications	Routine screening, restriction enzyme digestion, transformation, PCR and DNA sequencing

组分

Product	D6944-00	D6944-01	D6944-02
Purifications	10	100	300
FastFilter Mini Columns	10	100	300
HiBind® DNA Mini Columns	10	100	300
2 mL Collection Tubes	10	100	300
Solution I	3 mL	20 mL	60 mL
Solution II	3 mL	20 mL	60 mL
N3 Buffer	2.5 mL	10 mL	35 mL
GBT Buffer	6 mL	20 mL	2 × 20 mL
HBC Buffer	5 mL	25 mL	80 mL
DNA Wash Buffer	2.5 mL	25 mL	2 × 25 mL
Elution Buffer	2 mL	15 mL	30 mL
RNase A	Pre-added	100 μL	400 μL
User Manual	✓	✓	✓

流程

Spin Protocol

Pellet by Centrifugation
Resuspend and Lyse
Neutralize
Transfer Lysate to FastFilter Mini Column, nested in a HiBind® DNA Mini Column
Clear Lysate and Bind
Wash 2X
Dry
Elute

Vacuum/Spin Protocol

Pellet by Centrifugation
Resuspend and Lyse
Neutralize
Transfer Lysate to FastFilter Mini Column, nested in a HiBind® DNA Mini Column
Clear Lysate and Bind
Transfer to manifold
Wash 2X
Dry
Elute

上一篇： <D6948> Endo-Free Plasmid Mini Kit I 无内毒素质粒小量提取试剂盒I

下一篇： <D6929> Fastfilter Plasmid Mega Kit 快速法质粒超大量提取试剂盒

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