<D6950> Endo-Free Plasmid DNA Mini Kit II 无内毒素质粒小量提取试剂盒II

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无内毒素质粒小量提取试剂盒II

Endo-Free Plasmid DNA Mini Kit II

产品编号	产品名称	包装规格	目录价
D6950-00	无内毒素质粒小量提取试剂盒II	5T	100
D6950-01	无内毒素质粒小量提取试剂盒II	50T	675
D6950-02	无内毒素质粒小量提取试剂盒II	200T	2520

产品介绍

Plasmid isolated with traditional purification procedures normally contain high levels of endotoxins (also known as lipopolysaccharides or LPS) that can significantly interfere with transfection experiments downstream. The E.Z.N.A.® Endo-Free Plasmid DNA Mini Kit II integrates an efficient endotoxin removal step into the plasmid purification procedure to produce high-quality transfection grade (<1 EU/µg) plasmid for efficient transfection. The bacterial cells are lysed using the alkaline-SDS lysis method. The cleared cell lysate is then treated with ETR reagent to efficiently remove the endotoxins. After adjusting the binding condition, the cell lysate is applied into the HiBind® DNA column and purified DNA is eluted from the column membrane.

参数

FEATURES	SPECIFICATIONS
Downstream Application	Sensitive cell line transfection; gene therapy etc.
Starting material	5–15 mL LB culture with OD600 between 2 and 3; or equivalent
Plasmid type	High-copy, low-copy, cosmid DNA
Processing mode	Manual, centrifugation
Throughput	1 – 24
DNA binding technology	Silica Mini Spin Column II (2x capacity of mini spin column)
Lysate clearance method	Centrifugation
Processing time	<30 minutes
Yield	40–70 μg for high copy-number; 1–15 μg for low copy-number
Special note	Includes endotoxin removal step

组分

Endo-Free Plasmid Mini Kit II	D6950-00	D6950-01	D6950-02
Purifications	5	50	200
HiBind® DNA Mini Columns II	5	50	200
2 mL Collection Tubes	5	50	200
Solution I	5 mL	30 mL	120 mL
Solution II	5 mL	30 mL	120 mL
N3 Buffer	2.5 mL	15 mL	60 mL
ETR Binding Buffer	10 mL	80 mL	2 × 160 mL
ETR Wash Buffer	5 mL	30 mL	120 mL
HBC Buffer	5 mL	25 mL	80 mL
DNA Wash Buffer	2.5 mL	25 mL	3 × 25 mL
RNase A	Pre-added	100 μL	400 μL
Endo-Free Elution Buffer	2 mL	30 mL	60 mL
User Manual	✓	✓	✓

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