<D6904> Plasmid DNA Midi Kit 质粒中量提取试剂盒

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The E.Z.N.A. Plasmid Midi and Maxi Kits can isolate up to 250 μg and 1.2 mg of plasmid DNA from 50 mL and 200 mL bacterial cultures, respectively.

‍‍‍‍‍质粒中量提取试剂盒

Plasmid DNA Midi Kit

产品编号	产品名称	包装规格	目录价
D6904-00	质粒中量提取试剂盒	2T	140
D6904-01	质粒中量提取试剂盒	10T	500
D6904-03	质粒中量提取试剂盒	25T	1170

产品介绍

The E.Z.N.A. Plasmid Midi and Maxi Kits can isolate up to 250 μg and 1.2 mg of plasmid DNA from 50 mL and 200 mL bacterial cultures, respectively. These kits use a modified alkaline lysis method to lyse the cells and separate genomic DNA from plasmid DNA. Cellular debris are removed by centrifugation, and the protocol follows a simple bind, wash, and elute procedure to deliver high-quality

plasmid DNA. The system not only eliminates the time-consuming isopropanol precipitation step required by gravity flow columns but also the need for expensive accessories. Purified plasmid DNA is suitable for automated fluorescent DNA sequencing (typical reads exceed 800 bp), restriction enzyme digestion, ligation, PCR, in-vitro transcription, transformation, and other applications.

参数

FEATURES	SPECIFICATIONS
Downstream Application	Cloning, sequencing, transformation, PCR, restriction digestion, ligation, in vitro transcription etc.
Starting material	15–50 mL LB culture; Optimal Cell Mass (OD600 x mL culture) is 80–100.
Plasmid type	High-copy, low-copy, cosmid DNA
Processing mode	Manual (centrifugation or vacuum)
Throughput	1 – 24
DNA binding technology	Silica Midi Spin Column
Lysate clearance method	Centrifugation
Processing time	60 minutes
Yield	100–250 μg for high copy-number; 10–50 μg for low copy-number

组分

Plasmid DNA Midi Kit	D6904-00	D6904-03	D6904-04
Purifications	2	25	100
HiBind® DNA Midi Columns	2	25	100
15 mL Collection Tubes	2	25	100
Solution I	8 mL	70 mL	270 mL
Solution II	8 mL	70 mL	270 mL
Solution III	8 mL	100 mL	400 mL
HBC Buffer	5 mL	58 mL	250 mL
DNA Wash Buffer	5 mL	40 mL	200 mL
RNase A	25 μL	300 μL	1.2 mL
Elution Buffer	5 mL	60 mL	220 mL
User Manual	✓	✓	✓

流程

Centrifugation Protocol

Pellet by Centrifugation
Resuspend, Lyse, Neutralize
Clear Lysate
Transfer cleared lysate to HiBind® DNA Column
Bind
Wash 3X
Dry
Elute

Vacuum Protocol

Pellet by Centrifugation
Resuspend, Lyse, Neutralize
Clear Lysate
Transfer cleared lysate to HiBind® DNA Column
Bind
Wash 3X
Dry
Elute

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