<D6945> Plasmid DNA Mini Kit II 质粒小量提取试剂盒II

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This second-generation plasmid miniprep kit yields 40-70 µg of high-quality DNA in less than 30 minutes using the same format as the E.Z.N.A.® Plasmid DNA Mini Kit I.

质粒小量提取试剂盒II

‍‍‍‍Plasmid DNA Mini Kit II

产品编号	产品名称	包装规格	目录价
D6945-00	质粒中量提取试剂盒	5T	85
D6945-01	质粒中量提取试剂盒	50T	495
D6945-02	质粒中量提取试剂盒	200T	1800

产品介绍

This second-generation plasmid miniprep kit yields 40-70 µg of high-quality DNA in less than 30 minutes using the same format as the E.Z.N.A.® Plasmid DNA Mini Kit I. The increased DNA binding capacity of the HiBind® Mini Column II allows culture volumes of up to 15 mL to be used, thus bridging the gap between mini and midi prep protocols. Plasmid DNA can be readily and quickly isolated for most downstream applications such as routine screening, restriction enzyme digestion, and DNA sequencing.

参数

FEATURES	SPECIFICATIONS
Downstream Application	Cloning; sanger sequencing etc.
Starting material	5–15 mL LB culture with OD600 between 2 and 3; or equivalent
Plasmid type	High-copy, low-copy, cosmid DNA
Processing mode	Manual, centrifugation or vacuum
Throughput	1 – 24
DNA binding technology	Silica Mini Spin Column II (2x capacity of mini spin column)
Lysate clearance method	Centrifugation
Processing time	30 minutes
Yield	40–70 μg for high copy-number; 1–15 μg for low copy-number

组分

Plasmid Mini Kit II	D6945-00	D6945-01	D6945-02
Preps	5	50	200
HiBind® DNA Mini Columns II	5	50	200
2 mL Collection Tubes	5	50	200
Solution I	5 mL	30 mL	120 mL
Solution II	5 mL	30 mL	120 mL
Solution III	5 mL	40 mL	2 × 80 mL
HBC Buffer	5 mL	25 mL	80 mL
DNA Wash Buffer	2.5 mL	25 mL	3 × 25 mL
RNase A	Pre-Added	100 μL	400 μL
Elution Buffer	2 mL	30 mL	30 mL
User Manual	✓	✓	✓

流程

Spin Protocol

Pellet by Centrifugation
Resuspend and Lyse
Neutralize
Clear Lysate
Transfer Lysate to HiBind® DNA Mini Column
Bind
Wash 3X
Dry
Elute

Vacuum/Spin Protocol

Pellet by Centrifugation
Resuspend and Lyse
Neutralize
Clear Lysate
Transfer Lysate to HiBind® DNA Mini Column
Bind
Wash 3X
Dry
Elute

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