<D6943> Plasmid DNA Mini Kit I, (V-spin) 质粒小量提取试剂盒I

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he E.Z.N.A.® Plasmid DNA Mini Kit I is designed to isolate up to 25 µg of high-quality plasmid DNA from 1-5 mL bacterial cultures in less than 30 minutes.

‍‍质粒小量提取试剂盒I

Plasmid DNA Mini Kit I, (V-spin)

产品编号	产品名称	包装规格	目录价
D6943-01	质粒小量提取试剂盒I	100T	430
D6943-02	质粒小量提取试剂盒I	200T	720
D6943-03	质粒小量提取试剂盒I	500T	1650

产品介绍

The E.Z.N.A.® Plasmid DNA Mini Kit I is designed to isolate up to 25 µg of high-quality plasmid DNA from 1-5 mL bacterial cultures in less than 30 minutes. Plasmid DNA purification follows the alkaline-lysis method and is simplified with HiBind® Mini Column technology into three quick steps: Bind, Wash, and Elute. Purified plasmid DNA is immediately ready for a wide variety of downstream applications such as routine screening, restriction enzyme digestion, transformation, PCR and DNA sequencing.

The E.Z.N.A.® Plasmid DNA Mini Kit is available in two formats: Q-spin (D6942) and V-spin (D6943). D6942 (Q-spin) features columns that are capless, whereas D6943 (V-spin) includes columns that have a cap attached. The columns are otherwise identical in use and application and can be used in both vacuum or centrifugation protocols.

参数

FEATURES	SPECIFICATIONS
Downstream Application	Cloning, sequencing, transformation, PCR, restriction digestion, ligation, in vitro transcription etc.
Starting material	1–5 mL LB culture
Plasmid type	High-copy, low-copy, cosmid DNA
Processing mode	Manual (centrifugation or vacuum)
Throughput	1–24
DNA binding technology	Silica Mini Spin Column
Lysate clearance method	Centrifugation
Processing time	30 minutes
Yield	15–25 μg for high copy-number; 0.1–5 μg for low copy-number
Special note	The alternative for discontinued yeast plasmid kit

组分

Plasmid Mini Kit I	D6943-00	D6943-01	D6943-02	D6943-03
Preps	5	50	200	600
HiBind® DNA Mini Columns	5	50	200	600
2 mL Collection Tubes	5	50	200	600
Solution I	3 mL	20 mL	60 mL	200 mL
Solution II	3 mL	20 mL	60 mL	200 mL
Solution III	3 mL	20 mL	80 mL	250 mL
HBC Buffer	5 mL	25 mL	80 mL	250 mL
DNA Wash Buffer	2.5 mL	25 mL	3 × 25 mL	100 mL
RNase A	Pre-Added	100 μL	400 μL	1.5 mL
Elution Buffer	2 mL	15 mL	30 mL	100 mL
User Manual	✓	✓	✓	✓

流程

Spin Protocol

Pellet by Centrifugation
Resuspend and Lyse
Neutralize
Clear Lysate
Transfer Lysate to HiBind® DNA Mini Column
Bind
Wash 3X
Dry
Elute

Vacuum/Spin Protocol

Pellet by Centrifugation
Resuspend and Lyse
Neutralize
Clear Lysate
Transfer Lysate to HiBind® DNA Mini Column
Bind
Wash 3X
Dry
Elute

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