<R6812> HP Total RNA Kit HP组织RNA提取试剂盒

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The E.Z.N.A.® HP Total RNA Isolation Kit provides a rapid and easy method for RNA isolation from cultured cells or tissues. The kit includes RNA Homogenizer columns that integrate sample homogenization and genomic DNA elimination into one single step.

HP组织RNA提取试剂盒

HP Total RNA Kit


产品编号

产品名称

包装规格

目录价

R6812-00

   组织RNA提取试剂盒     

5T

150

R6812-01

组织RNA提取试剂盒  

50T

1200

R6812-02

 组织RNA提取试剂盒   

200T

3800


产品介绍

The E.Z.N.A.® HP Total RNA Isolation Kit provides a rapid and easy method for RNA isolation from cultured cells or tissues. The kit includes RNA Homogenizer columns that integrate sample homogenization and genomic DNA elimination into one single step. The RNA purification process is simplified with HiBind® Mini Column technology and can be accomplished in 25 minutes. This convenient spin-column format requires no phenol or chloroform, avoids time-consuming steps such as CsCl gradient ultracentrifugation, precipitation with isopropanol or LiCl and allows for multiple samples to be processed in parallel. RNA purified using the E.Z.N.A.® HP Total RNA method is ready for applications such as RT-PCR, RT-qPCR, Northern blotting, next-generation sequencing, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.


参数

FEATURES

SPECIFICATIONS

Downstream Application

RT-PCR, RT-qPCR, Northern blotting, next-generation sequencing, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.

Starting Material

Cultured cells and tissues

Starting Amount

1 × 10⁷ eukaryotic cells or 25–30 mg tissue

Elution Volume

40–70 µL

RNA Binding Technology

Silica Mini spin column

Processing Mode

Manual (centrifugation or vacuum)

Throughput

1 – 24

Final Purified Product

Total RNA (> 200 nt)

RNA Yield

Up to 100 µg

Processing Time

25 min

Special Notes

RNA Homogenizer Column that integrates sample homogenization and gDNA elimination into a single step


组分

Product

R6812-00

R6812-01

R6812-02

Preparations

5

50

200

HiBind® RNA Mini Columns

5

50

200

RNA Homogenizer Mini Columns

5

50

200

2 mL Collection Tubes

10

100

400

GTC Lysis Buffer

5 mL

40 mL

150 mL

RNA Wash Buffer I

5 mL

50 mL

200 mL

RNA Wash Buffer II

5 mL

12 mL

50 mL

Nuclease-free Water

2 mL

15 mL

60 mL

User Manual


流程

  1. Add GTC Lysis Buffer and Lyse

  2. Transfer Lysate to RNA Homogenizer Mini Column

  3. Add Ethanol

  4. Apply Sample to Column

  5. Wash 3X

  6. Dry

  7. Elute