HP组织RNA提取试剂盒
HP Total RNA Kit
产品编号 | 产品名称 | 包装规格 | 目录价 |
R6812-00 | 组织RNA提取试剂盒 | 5T | 150 |
R6812-01 | 组织RNA提取试剂盒 | 50T | 1200 |
R6812-02 | 组织RNA提取试剂盒 | 200T | 3800 |
产品介绍
The E.Z.N.A.® HP Total RNA Isolation Kit provides a rapid and easy method for RNA isolation from cultured cells or tissues. The kit includes RNA Homogenizer columns that integrate sample homogenization and genomic DNA elimination into one single step. The RNA purification process is simplified with HiBind® Mini Column technology and can be accomplished in 25 minutes. This convenient spin-column format requires no phenol or chloroform, avoids time-consuming steps such as CsCl gradient ultracentrifugation, precipitation with isopropanol or LiCl and allows for multiple samples to be processed in parallel. RNA purified using the E.Z.N.A.® HP Total RNA method is ready for applications such as RT-PCR, RT-qPCR, Northern blotting, next-generation sequencing, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.
参数
FEATURES | SPECIFICATIONS |
Downstream Application | RT-PCR, RT-qPCR, Northern blotting, next-generation sequencing, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation. |
Starting Material | Cultured cells and tissues |
Starting Amount | 1 × 10⁷ eukaryotic cells or 25–30 mg tissue |
Elution Volume | 40–70 µL |
RNA Binding Technology | Silica Mini spin column |
Processing Mode | Manual (centrifugation or vacuum) |
Throughput | 1 – 24 |
Final Purified Product | Total RNA (> 200 nt) |
RNA Yield | Up to 100 µg |
Processing Time | 25 min |
Special Notes | RNA Homogenizer Column that integrates sample homogenization and gDNA elimination into a single step |
组分
Product | R6812-00 | R6812-01 | R6812-02 |
Preparations | 5 | 50 | 200 |
HiBind® RNA Mini Columns | 5 | 50 | 200 |
RNA Homogenizer Mini Columns | 5 | 50 | 200 |
2 mL Collection Tubes | 10 | 100 | 400 |
GTC Lysis Buffer | 5 mL | 40 mL | 150 mL |
RNA Wash Buffer I | 5 mL | 50 mL | 200 mL |
RNA Wash Buffer II | 5 mL | 12 mL | 50 mL |
Nuclease-free Water | 2 mL | 15 mL | 60 mL |
User Manual | ✓ | ✓ | ✓ |
流程
Add GTC Lysis Buffer and Lyse
Transfer Lysate to RNA Homogenizer Mini Column
Add Ethanol
Apply Sample to Column
Wash 3X
Dry
Elute