<R6834> Total RNA Kit I 总RNA提取试剂盒I

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The E.Z.N.A. Total RNA Kit I provides a simple and rapid method for the isolation of up to 100 μg total RNA from cultured eukaryotic cells and soft tissues.

总RNA提取试剂盒I

Total RNA Kit I

产品编号	产品名称	包装规格	目录价
R6834-00	总RNA提取试剂盒I	5T	135
R6834-01	总RNA提取试剂盒I	50T	900
R6834-02	总RNA提取试剂盒I	200T	3500

产品介绍

The E.Z.N.A. Total RNA Kit I provides a simple and rapid method for the isolation of up to 100 μg total RNA from cultured eukaryotic cells and soft tissues. Multiple samples of up to 1 x 107 eukaryotic cells or 30 mg of tissue can be processed in parallel in fewer than 20 minutes. Many different sample types can be used, including kidney, liver, heart, spleen, lung, pancreas, thymus, HeLa cells, HEK-293 cells, and HINT3T3 cells. After the homogenization process, samples are applied to the HiBind® RNA Mini Column which binds total RNA. Cellular debris and other contaminants are effectively washed away after a few quick wash steps. Total RNA greater than 200 nt is isolated using this kit. Purified RNA can be used in many downstream applications such as RT-PCR, qRT-PCR, Northern blotting, nuclease protection assay, microarrays, in-vitro translation, and Next Generation Sequencing.

参数

FEATURES	SPECIFICATIONS
Downstream Application	PCR, qPCR, real-time RT-PCR, microarray, Northern blot, poly-A purification
Elution Volume	40–70 µL
Starting Material	Cultured eukaryotic cells and soft tissues
Starting Amount	< 1 x 10⁷ cells or 30 mg tissue
RNA Yield	Up to 100 µg
Processing Mode	Manual, centrifugation/vacuum
Throughput	1 – 24
RNA Binding Technology	Silica Mini spin column
Binding Capacity	100 µg

组分

Product	R6834-00	R6834-01	R6834-02	R6834-03
Preparations	5	50	200	600
HiBind® RNA Mini Columns	5	50	200	600
2 mL Collection Tubes	10	100	400	1200
TRK Lysis Buffer	5 mL	40 mL	150 mL	500 mL
RNA Wash Buffer I	5 mL	50 mL	200 mL	600 mL
RNA Wash Buffer II	5 mL	12 mL	50 mL	200 mL
Nuclease-free Water	2 mL	30 mL	60 mL	125 mL
User Manual	✓	✓	✓	✓

流程

Add TRK Lysis Buffer and Lyse
Homogenize
Add Ethanol
Apply Sample to Column
Wash 3X
Dry
Elute

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