产品介绍

Mag-Bind® Plant DNA DS 96 Kit allows rapid and reliable isolation of high-quality genomic DNA from plants and other tissues that are particularly difficult to lyse or very high in polysaccharide content. The lysis and binding buffers are specifically designed to minimize co-purification of polysaccharides and polyphenols. Up to 96 samples of 50 mg wet tissue (or 15 mg dry tissue) can be processed in parallel in less than 1 hour.

The system combines Omega Bio-tek’s E.Z.N.A.® buffer chemistry, which eliminates the need for organic solvents, with the convenience of Mag-Bind® particles to eliminate polysaccharides, phenolic compounds, and enzyme inhibitors from plant tissue lysates. This kit is designed for manual or fully automated high-throughput preparation of genomic, chloroplast, and mitochondrial DNA. Purified DNA is suitable for PCR, restriction digestion, Next Generation Sequencing, and hybridization applications. There are no organic extractions, thereby reducing consumables and decreasing hands-on time to allow multiple samples to be processed in parallel.

参数

组分

流程

• Homogenize/Lyse

• Transfer Supernatant, Adjust Binding Conditions

• Bind DNA with Magnetic Beads

• Concentrate Magnetic Beads

• Wash

• Concentrate Magnetic Beads

• Dry

• Elute

• Transfer Product