磁珠法石蜡包埋组织DNA提取试剂盒
Mag-Bind FFPE DNA Kit
产品编号 | 产品名称 | 包装规格 | 目录价 |
M6957-01 | 磁珠法石蜡包埋组织DNA提取试剂盒 | 50T | 3250 |
M6958-01 | 磁珠法石蜡包埋组织DNA提取试剂盒 | 4 x 96 | 17390 |
产品介绍
The Mag-Bind® FFPE DNA 96 Kit is designed for rapid and reliable isolation of DNA from formalin-fixed, paraffin-embedded (FFPE) tissue. The specially formulated buffers reverse crosslinking and release short and long DNA fragments. Mag-Bind® Particles allow for automation-friendly extraction from FFPE tissue in under 6 hours. Purified DNA is suitable for downstream applications including SNP analysis, sequencing, and genotyping. This system is fully compatible on Hamilton Star, Beckman Coulter Biomek® FX, Biomek® 2000, Tecan Fluent™, Thermo KingFisher® Flex, and other workstations.
参数
FEATURES | SPECIFICATIONS |
Starting Amount | 3–8 paraffin sample sections between 5–10 μm |
Starting Material | FFPE tissue |
Elution Volume | 30–50 μL |
Technology | Magnetic beads |
Processing Mode | Automated, Manual |
Throughput | 96 |
组分
Mag-Bind®FFPE DNA 96 Kit | M6958-00 | M6958-01 |
Preparations | 1 × 96 preps | 4 × 96 preps |
Mag-Bind® Particles CH | 3.3 mL | 14 mL |
FTL2 Buffer | 30 mL | 110 mL |
MB4 Buffer | 75 mL | 250 mL |
MPWP Buffer | 30 mL | 125 mL |
DNA Wash Buffer | 50 mL | 200 mL |
LPA | 1.1 mL | 4.4 mL |
Proteinase K Solution | 2.2 mL | 9 mL |
Elution Buffer | 30 mL | 60 mL |
User Manual | ✓ | ✓ |
流程
Heat Incubate for Lysis / Decrosslink at 90℃
Transfer Supernatant / Adjust Binding Conditions
Bind Nucleic Acids with Magnetic Beads
Concentrate Magnetic Beads
Wash
Concentrate Magnetic Beads
Dry
Elute
Concentrate Magnetic Beads / Transfer Product