<D6492> Cycle Pure Kit(V-spin) PCR产物纯化试剂盒

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PCR产物纯化试剂盒

Cycle Pure Kit(V-spin)

产品编号	产品名称	包装规格	目录价
D6492-01	PCR产物纯化试剂盒	100T	380
D6492-02	PCR产物纯化试剂盒	200T	580
D6492-03	PCR产物纯化试剂盒	500T	1400

产品介绍

The E.Z.N.A.® Cycle Pure Kit is a convenient system for the fast and reliable purification of PCR products. The E.Z.N.A.® Cycle Pure Kit uses HiBind® technology to recover DNA bands from 100 bp to 10 kb free of oligonucleotides, nucleotides, and polymerase with yields exceeding 80%. The binding conditions of the HiBind® DNA Mini Columns are adjusted by the addition of a specially formulated buffer before adding the sample. Following a rapid wash step, DNA is eluted with deionized water or a low salt buffer. Purified DNA can be directly used for most downstream applications include T-A ligations, PCR sequencing, restriction enzyme digestion, or various labeling reactions.

参数

FEATURES	SPECIFICATIONS
Downstream application	T-A ligations, sequencing, restriction enzyme digestion, and various other labeling reactions
Elution volume	30–50 μL
Starting material	ssDNA, dsDNA, PCR products
Starting amount	5–50 μL
DNA recovered	>80% recovery, 100 bp to 10 kb
Processing mode	Manual (centrifugation or vacuum)
Throughput	1–24
DNA binding technology	Silica mini spin column
Processing time	10 minutes
Special Notes	For clean-up of DNA fragments 200 bp, please follow the protocol modification outlined in the product manual

组分

Product Number	D6492-00	D6492-01	D6492-02	D6492-03
Preparations	5	50	200	600
HiBind® DNA Mini Columns	5	50	200	600
2 mL Collection Tubes	5	50	200	600
CP Buffer	5 mL	40 mL	150 mL	3 × 120 mL
Elution Buffer	15 mL	30 mL	30 mL	35 mL
DNA Wash Buffer	2.5 mL	25 mL	3 × 25 mL	200 mL
User Manual	✓	✓	✓	✓

流程

Add CP Buffer
Transfer Lysate and Bind
Wash
Dry
Elute

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