<D6294> MicroElute Cycle-Pure&Gel Extraction Kit 微量胶回收试剂盒上海诺宁生物科技有限公司

<D6294> MicroElute Cycle-Pure&Gel Extraction Kit 微量胶回收试剂盒

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The E.Z.N.A.® family of products is an innovative system that radically simplifies the extraction and purification of nucleic acids from a variety of sources.

‍‍‍微量胶回收试剂盒

MicroElute Cycle-Pure&Gel Extraction Kit

产品编号	产品名称	包装规格	目录价
D6294-00	微量胶回收试剂盒	5T	100
D6294-01	微量胶回收试剂盒	50T	400
D6294-02	微量胶回收试剂盒	200T	1500

产品介绍

The E.Z.N.A.® family of products is an innovative system that radically simplifies the extraction and purification of nucleic acids from a variety of sources. The key to this system is the HiBind® matrix that specifically, but reversibly, binds DNA or RNA under optimized conditions allowing proteins and other contaminants to be removed. Nucleic acids are easily eluted with deionized water or a low salt buffer. The MicroElute® Clean Up system, designed for rapid DNA clean up, includes:

• MicroElute® Cycle-Pure – for direct purification of double or single stranded PCR products (100 bp – 10 kb) from amplification reactions
• MicroElute® Gel Extraction – for extraction of DNA fragments from standard, or low-melt agarose gels in TAE (Tris-acetate/EDTA) or TBE (Tris-borate/EDTA) buffer

参数

FEATURES	SPECIFICATIONS
Downstream application	Cloning, In Vitro Transcription, Nucleic Acid Labeling, PCR, Real-Time Quantitative PCR (qPCR), Sequencing, Southern Blotting
Elution volume	10–15 μL
Starting material	Agrose gel slice
Starting amount	Up to 10 μg DNA
DNA recovered	>90% recovery, 70 bp to 20 kb
Processing mode	Manual, centrifugation/vacuum
Throughput	1
DNA binding Technology	Silica MicroElute spin column
Binding capacity	10 μg
Processing time	<15 minutes
Special note	Low elution volume

组分

Product	D6294-00	D6294-01	D6294-02
Purifications	5	50	200
MicroElute® LE DNA Columns	5	50	200
2 mL Collection Tubes	5	50	200
CP Buffer	5 mL	30 mL	120 mL
XP2 Binding Buffer	5 mL	30 mL	120 mL
Buffer HB	5 mL	25 mL	25 mL
SPW Wash Buffer	2.5 mL	25 mL	3 × 25 mL
Elution Buffer	2 mL	30 mL	30 mL
User Manual	✓	✓	✓

流程

Excise Gel Slice
Dissolve Gel
Bind DNA
Wash
Dry Spin
Elute DNA