<D6900>M13 DNA Mini Kit M13 DNA提取试剂盒

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Omega Bio-tek's E.Z.N.A.® M13 DNA Kits are designed to purify up to 10 µg of single stranded DNA from up to 3 mL of phage supernatant. Yields of single stranded DNA obtained using E.Z.N.A.® M13 DNA Kits are around 3-10 µg and reproducible when the isolations are performed from the same culture.


M13 DNA提取试剂盒

M13 DNA Mini Kit


产品编号

产品名称

包装规格

目录价

D6900-00

     M13 DNA提取试剂盒     

5T

100

D6900-01

M13 DNA提取试剂盒

50T

495

D6900-02

M13 DNA提取试剂盒

200T

1800


产品介绍

The E.Z.N.A.® family of products is an innovative system that radically simplifies the extraction and purification of nucleic acids from a variety of sources. The key to this system is Omega Bio-tek's HiBind® matrix that specifically, but reversibly, binds DNA or RNA under optimal conditions while allowing proteins and other contaminants to be removed. The nucleic acids bound to the HiBind® matrix are easily eluted with deionized water or low salt buffer. DNA and RNA are suitable for many downstream applications.

Omega Bio-tek's E.Z.N.A.® M13 DNA Kits are designed to purify up to 10 µg of single stranded DNA from up to 3 mL of phage supernatant. Yields of single stranded DNA obtained using E.Z.N.A.® M13 DNA Kits are around 3-10 µg and reproducible when the isolations are performed from the same culture.


参数

FEATURES

SPECIFICATIONS

Downstream Application

Single-stranded M13 DNA

Starting material

3 mL phage supernatant

Plasmid type

Single-stranded M13 DNA

Processing mode

Manual, centrifugation

Throughput

 24

DNA binding technology

Silica Mini Spin Column

Lysate clearance method

Not needed

Yield

310 μg single-stranded DNA


组分

Product

D6900-00

D6900-01

Purifications

5 preps

50 preps

HiBind® M13 DNA Mini Column

5

50

2 mL Collection Tubes

5

50

MPB Buffer

2 mL

20 mL

MPX Buffer

8 mL

80 mL

SPW Buffer

5 mL

25 mL

Elution Buffer

15 mL

30 mL

User Manual


流程

  1. Precipitation

  2. Take Supernatant

  3. Add MPC

  4. Bind

  5. Wash

  6. Dry Spin

  7. Elute