MCLA (Chemiluminescent Probe) 超氧化物化学发光探针

产品简介：

MCLA，又称为6-(4-Methoxyphenyl)-2-methyl-3,7-dihydroimidazo[1,2-a]pyrazin-3(7H)-one hydrochloride，能够与超氧化物阴离子（O2–）或单线态氧（1O2)反应产生化学发光。MCLA与超氧化物可逆反应形成一加合物，该加合物的不可逆衰变产生冷光（~465nm），此反应表观速率常数是~105M–1s–1。MCLA化学发光是灵敏的超氧化物检测手段，最显著的应用是白细胞功能研究。

产品特性：

1）CAS NO.：128322-44-1

2）同义名：2-Methyl-6-(4-methoxyphenyl)-3,7-dihydroimidazo[1,2-a]pyrazin

-3(7H)-one hydrochloride, 6-(4-Methoxyphenyl)-2-methyl-3,7-dihydroimidazo[1,2

-a]pyrazin-3(7H)-one hydrochloride, Cypridina Luciferin methoxy-analogue, MCLA

3）分子式：C14H13N3O2· HCl 

4）分子量：291.73

5）纯度：≥98.0%

6）Emmax：~465 nm (Chemiluminescence: in 0.1 M Tris pH 8.5; after addition of H2O2)

7）外观：白色至浅黄色固体

8）溶解性：溶于水，DMSO，DMF

9）化学结构图：

保存条件: 

20ºC避光干燥保存，至少1年有效。

产品使用：（仅供参考）

Prepare reaction buffer: 5 µM MCLA + buffer

Buffer examples:  

for Cytochrome C: 50 mM Tris-Cl, pH 7.8, 0.1 mM EDTA, 7 mM sodium succinate .

for mitochondria proteins: 125 mM KCl, 10 mM HEPES, 5 mM MgCl2, 2 mM K2HPO4

Conduct reaction in 100 µl of reaction buffer - Light emission is detected and quantified using a microplate luminometer with opaque (white) 96-well plates. The photomultiplier can be set to default with an integration time of 1000 ms. The MCLA signal is quantified as an integral of 20 s to 60 s of continuous measurement. - A positive control can be prepared with the xanthine/xanthine oxidase system: 1 mM xanthine + 0.1 unit/ml xanthine oxidase causes a ~100-fold increase in chemiluminescent signal as compared with xanthine or xanthine oxidase alone, which did not differ significantly from reaction buffer alone. Addition of SOD (CuZn-SOD from erythrocytes) decreases the MCLA signal by over 98%.

注意事项：

1.      为了您的安全和健康，请穿实验服并戴一次性手套操作。