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X-gluc特价促销,gus基因染色首选
发表日期:2012-5-30

                                                      X-gluc特价促销

In order to identify transformed cells or plants that have been growing on a selective medium, it is necessary to have an easily assayable reporter gene.
(X-glu, X-glcA) 5-Bromo-4-chloro-3-indoxyl-beta-D-glucuronide cyclohexylammonium salt
Sold as 1 g units
MOLECULAR BIOLOGY GRADE:C14 H13 Br Cl N O7? C6 H13 N
M.W.:521.79
Purity: greater than 99% (HPLC)
Thin Layer Chromatogram: single spot
Biological Activity: indigo-blue assay
Elemental Analysis: Agrees with theoretical
#118alues
CAS RN: [18656-96-7]
GUS Staining of Pollen:操作步骤
1. Add 200μl of acetone, vortex briefly.
2. Remove acetone and pipette into flat-bottom 96-well plate (polystyrene)
3. Centrifuge plate at 3000 x g for 3-5 minutes.
4. Invert plate carefully and blot dry with paper towels
5. Add 40μl of X-Gluc stain solution to each well (multi-pipettor) and incubate at
37°C overnight.
X-Gluc Solution:溶液配制
1 20 mg/ml X-Gluc concentration. e.g. For a 1.5 ml tube (microfuge tube)
dissolve 30 mg (.03 g) of X-Gluc in 1.5 ml DMSO (dimethyl sulfoxide).
2 Cover with foil.
3 Vortex well.
4 Keep at -20°C.
X-Gluc stain solution:解决方法
*Note: 4ml of stain solution are necessary to stain 1 96-well plate.
References using GBT X-Gluc:相关论文
1. Ourmanov, I. et al. (2009). Improved Survival in Rhesus Macaques Immunized with Modified Vaccinia Virus. Journal of Virology, 83(11), 5388-5400.
2. Coque, L. et al. (2008). Transcription of ENOD8 in Medicago truncatula Nodules Directs ENOD8 Esterase to Developing and Mature Symbiosomes. Molecular Plant-Microbe Interactions, 21(4), 404-410.
3. Kim, H., & Woloshuk, C.P. (2008). Role of AREA, a regulator of nitrogen metabolism, during colonization of maize kernels and fumonisin biosynthesis in Fusarium verticillioides. Fungal Genetics and Biology, 45 (6), 947-953.
4. Scarpeci, T.E. (2008). Generation of superoxide anion in chloroplasts of Arabidopsis thaliana during active photosynthesis: a focus on rapidly induced genes . Plant Molecular Biology, 66(4), 361-378.
5. Geddie, M.L., & Matsumura, I. (2007). Antibody-Induced Oligomerization and Activation of an Engineered Reporter Enzyme. Journal of Molecular Biology, 369 (4), 1052-1059.
6. Ko et al. (2004). In Vitro Cellular and Developmental Biology- Plant, 40 (6). 552-558.
7. Curtis et al. (2000). Annals of Botany, 86. 63-71.
8. Ingram et al. (2000).Plant Cell, Tissue and Organ Culture, 57. 207-210.

订购信息:

品牌                           货号                    规格                        促销价

GoldBio                   G1281                   1g                           1800


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